Development of a Microsatellite Multiplex for Paternity Testing in Uruguayan Corriedale Sheep

Authors

  • Pablo Peraza Unidad de Biotecnología Animal, Instituto Nacional de Investigación Agropecuaria. Ruta 48 km 10, Rincón del Colorado, Canelones, Uruguay
  • Gonzalo Rincón Animal Science Department, Universidad de California. Davis, USA.
  • Olga Ravagnolo Programa de Carne y Lana. Instituto Nacional de Investigación Agropecuaria.
  • Marco Dalla Rizza Unidad de Biotecnología Animal, Instituto Nacional de Investigación Agropecuaria. Ruta 48 km 10, Rincón del Colorado, Canelones, Uruguay.
  • Lucy Kelly Unidad de Biotecnología Animal, Instituto Nacional de Investigación Agropecuaria. Ruta 48 km 10, Rincón del Colorado, Canelones, Uruguay.

DOI:

https://doi.org/10.31285/AGRO.17.523

Keywords:

DNA analysis, sheep, STR

Abstract

The Corriedale breed represents 70% of Uruguayan sheep stock, therefore it is important to guarantee accuracy in paternity assessment through DNA analysis, to contribute to genealogy recording and selection programs for economically relevant traits of the breed. In this study, a multiplex of 10 microsatellites (Short Tandem Repeats, STR) recommended from the ISAG (International Society of Animal Genetics) panel was developed. With this multiplex, a population of 156 Corriedale sheep was analyzed. The aim of this study was to evaluate the multiplex according to genetic variability of allele frequencies, for assigning parents through the their Probability of Exclusion (PE) values. The STR showed a relatively high variability according to the average Heterozygosity Index (HObs=0.661), the number of alleles observed (N=8.6), and the Polymorphic Information Content (PIC=0.614). In four ot the 10 STR (FCB20, TGLA53, MCM527 and MCM130) there was a loss of Hardy-Weinberg equilibrium due to the significant decrease of heterozygotes, with a consequent reduction of genetic variability. As a result, combined exclusion probability presented a value of 0.960. We conclude that in order to improve the accuracy in paternity diagnostic the multiplex could include a greater number of STR, given the low genetic variability present in the parents.

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Published

2013-06-01

How to Cite

1.
Peraza P, Rincón G, Ravagnolo O, Dalla Rizza M, Kelly L. Development of a Microsatellite Multiplex for Paternity Testing in Uruguayan Corriedale Sheep. Agrocienc Urug [Internet]. 2013 Jun. 1 [cited 2024 Jun. 15];17(1):114- 119. Available from: https://agrocienciauruguay.uy/index.php/agrociencia/article/view/523

Issue

Section

Animal production and pastures
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