Polyphenols and minerals in propolis from different agricultural regions of Uruguay

Authors

DOI:

https://doi.org/10.31285/AGRO.28.1240

Keywords:

propolis, mineral profile, polyphenol profile, vegetable description

Abstract

The broad spectrum of plant origin of propolis makes them very heterogeneous in terms of their chemical composition. This fact conditions the potential use in medicine. Among the main bioactive components, polyphenols and minerals are related to different medicinal properties attributed to propolis. This study aims to identify and quantify the presence of 15 polyphenols and 8 minerals, in a collection of georeferenced propolis from 14 locations in Uruguay. Polyphenols in ethanolic extracts of propolis were identified and quantified by HPLC, while minerals were studied by atomic absorption spectroscopy in crude propolis. The plant environment was characterized using information on the productive use of the soil. The three main polyphenols found in all locations were chrysin, galangin and pinocembrin. All polyphenols analyzed were found, except rutin, which was not detected in any sample. Regarding the concentration of polyphenols, we can divide Uruguay into two regions: the southwest with high concentrations of 125 g/kg, and the northeast with concentrations of 16 g/kg. All minerals were identified. The variations between localities and seasons rule out the use of minerals for a determination of geographical origin. The description of the plant environment was partially adjusted with the types of propolis. Concentrations found of polyphenols and minerals are within the values reported in the world. Studies on the high polyphenolic content of propolis from the southwestern region should continue due to its potential medicinal use.

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References

Ali AM, Kunugi H. Propolis, bee honey, and their components protect against coronavirus disease 2019 (COVID-19): a review of in silico, In Vitro, and clinical studies. Molecules. 2021;26(5):1232. Doi: 10.3390/molecules26051232.

Andrade JKS, Denadai M, de Oliveira CS, Nunes ML, Narain N. Evaluation of bioactive compounds potential and antioxidant activity of brown, green and red propolis from Brazilian northeast region. Food Res Int. 2017;101:129-38. Doi: 10.1016/j.foodres.2017.08.066.

Anjum SI, Ullah A, Khan KA, Attaullah M, Khan H, Ali H, Bashir MA, Tahir M, Ansari MJ, Ghramh HA, Adgaba N, Dash CK. Composition and functional properties of propolis (bee glue): a review. Saudi J Biol Sci. 2019;26(7):1695-703. Doi: 10.1016/j.sjbs.2018.08.013.

Arslan M, Sevgiler Y, Güven C, Murathan ZT, Erbil N, Yıldırım D, Büyükleyla M, Karadaş Ş, Çelik R, Rencüzoğulları E. Chemical and biological characteristics of propolis from Apis mellifera caucasica from the Ardahan and Erzurum provinces of Turkey: a comparative study. Arh Hig Rada Toksikol. 2021;72(1):53-69. Doi: 10.2478/aiht-2021-72-3492.

Ballinger JT, Shugar GJ. Chemical technicians' ready reference handbook. 5th ed. New York: McGrawHill; 2011. 704p.

Bankova V, Popova M, Trusheva B. Propolis volatile compounds: chemical diversity and biological activity: a review. Chem Cent J. 2014;8:28. Doi: 10.1186/1752-153X-8-28.

Bankova V, Trusheva B, Popova M. Propolis extraction methods: A review. J Apic Res. 2021;60(5):734-43. Doi: 10.1080/00218839.2021.1901426.

Bankova VS, de Castro SL, Marcucci MC. Propolis: recent advances in chemistry and plant origin. Apidologie. 2000;31(1):3-15. Doi: 10.1051/apido:2000102.

Blondel VD, Guillaume JL, Lambiotte R, Lefebvre E. Fast unfolding of communities in large networks. J Stat Mech Theory Exp. 2008;P10008. Doi: 10.1088/1742-5468/2008/10/P10008.

Bonvehí JS, Bermejo FJ. Element content of propolis collected from different areas of South Spain. Environ Monit Assess. 2013;185(7):6035-47. Doi: 10.1007/s10661-012-3004-3.

Bossi J, Gaucher C. Geología del Uruguay. Montevideo: Polo; 2014. 2v.

Burdock GA. Review of the biological properties and toxicity of bee propolis (propolis). Food Chem Toxicol. 1998;36(4):347-63. Doi: 10.1016/s0278-6915(97)00145-2.

Calegari MA, Prasniewski A, Silva CD, Sado RY, Maia FMC, Tonial LMS, Oldoni TLC. Propolis from Southwest of Parana produced by selected bees: Influence of seasonality and food supplementation on antioxidant activity and phenolic profile. An Acad Bras Cienc. 2017;89(1):45-55. Doi: 10.1590/0001-3765201620160499.

Cantarelli MA, Camiña JM, Pettenati E, Marchevsky E, Pellerano R. Trace mineral content of Argentinean raw propolis by neutron activation analysis (NAA): Assessment of geographical provenance by chemometrics. LWT. 2011;44(1):256-60. Doi: 10.1016/j.lwt.2010.06.031.

Coelho J, Falcao SI, Vale N, Almeida-Muradian LB, Vilas-Boas M. Phenolic composition and antioxidant activity assessment of southeastern and south Brazilian propolis. J Apic Res. 2017;56(1):21-31. Doi: 10.1080/00218839.2016.1277602.

Cracco P, Cabrera C, Cadenazzi M, Galietta G, Moreni A, Santos E, Zaccari F. Uruguayan honey from different regions, characterization and origin markers. Agrocienc Urug. 2022;26(1):e947. Doi: 10.31285/AGRO.26.947.

Cracco P, Cabrera MC, Galietta G. Physicochemical characterization of georeferenced propolis from 14 locations of Uruguay. Agrocienc Urug. 2023;27:e1181. Doi: 10.31285/AGRO.27.1181.

Cvek J, Medić-Šarić M, Vitali D, Vedrina-Dragojević I, Šmit Z, Tomić S. The content of essential and toxic elements in Croatian propolis samples and their tinctures. J Apic Res. 2008;47(1):35-45.

Di Rienzo JA, Casanoves F, Balzarini MG, González L, Tablada M, Robledo CW. InfoStat [Internet]. Version 2008. Córdoba: Universidad Nacional de Córdoba, Facultad de Ciencias Agropecuarias; 2008 [cited 2023 Dec 28]. Available from: https://bit.ly/3dDvIyu

Elbatreek MH, Mahdi I, Ouchari W, Mahmoud MF, Sobeh M. Current advances on the therapeutic potential of pinocembrin: An updated review. Biomed Pharmacother. 2023;157:114032. Doi: 10.1016/j.biopha.2022.114032.

Galeotti F, Capitani F, Fachin A, Volpi N. Recent advances in analytical approaches for the standardization and quality of polyphenols of propolis. J Med Plant Res. 2019;13(19):487-500.

Ghisalberti EL. Propolis: a review. Bee world. 1979;60(2):59-84.

Google Earth [Internet]. Mountain View: Google; 2001- [cited 2023 Dec 28]. Available from: https://www.google.es/intl/es/earth/index.html

Guler HI, Tatar G, Yildiz O, Belduz AO, Kolayli S. Investigation of potential inhibitor properties of ethanolic propolis extracts against ACE-II receptors for COVID-19 treatment by molecular docking study. Arch Microbiol. 2021;203(6):3557-64. Doi: 10.1007/s00203-021-02351-1.

Kumazawa S, Hayashi K, Kajiya K, Ishii T, Hamasaka T, Nakayama T. Studies of the constituents of Uruguayan propolis. J Agric Food Chem. 2002;50(17):4777-82. Doi: 10.1021/jf020279y.

Kurek-Górecka A, Keskin Ş, Bobis O, Felitti R, Górecki M, Otręba M, Stojko J, Olczyk P, Kolayli S, Rzepecka-Stojko A. Comparison of the Antioxidant Activity of Propolis Samples from Different Geographical Regions. Plants (Basel). 2022;11(9):1203. Doi: 10.3390/plants11091203.

Kuropatnicki AK, Szliszka E, Krol W. Historical aspects of propolis research in modern times. Evid Based Complement Alternat Med. 2013;2013:964149. Doi: 10.1155/2013/964149.

Laboratory Sample Preparation. In: Multi-Agency Radiological Laboratory Analytical Protocols Manual (MARLAP). Vol. 2. Washington: United States Environmental Protection Agency; 2004. 44p.

Liu Y, Liang X, Zhang G, Kong L, Peng W, Zhang H. Galangin and pinocembrin from propolis ameliorate insulin resistance in HepG2 cells via regulating Akt/mTOR Signaling. Evid Based Complement Alternat Med. 2018;2018:7971842. Doi: 10.1155/2018/7971842.

Lombardo A. Los árboles cultivados en los paseos públicos. Montevideo: Consejo Departamental de Montevideo; 1958. 290p.

Lombardo A. Los árboles cultivados en los paseos públicos. Montevideo: IMM; 1979. 306p.

Mazimba O. Umbelliferonae: sources, chemistry and bioactivities review. Bull Fac Pharm Cairo Univ. 2017;55(2):223-32. Doi: 10.1016/j.bfopcu.2017.05.001.

Miłek M, Ciszkowicz E, Tomczyk M, Sidor E, Zaguła G, Lecka-Szlachta K, Pasternakiewicz A, Dżugan M. The study of chemical profile and antioxidant properties of poplar-type Polish Propolis considering local flora diversity in relation to antibacterial and anticancer activities in human breast cancer cells. Molecules. 2022;27(3):725. Doi: 10.3390/molecules27030725.

Ministerio de Ganadería, Agricultura y Pesca (UY). Mapa integrado de cobertura/uso del suelo del Uruguay año 2018 [Internet]. Montevideo: MGAP; 2018 [cited 2023 Dec 28]. Available from. https://www.gub.uy/ministerio-ganaderia-agricultura-pesca/comunicacion/publicaciones/mapa-integrado-coberturauso-del-suelo-del-uruguay-ano-2018

Molnár S, Mikuska K, Patonay K, Sisa K, Daood HG, Némedi E, Kiss A. Comparative studies on polyphenolic profile and antimicrobial activity of propolis samples selected from distinctive geographical areas of Hungary. Food Sci Technol Int. 2017;23(4):349-57. Doi: 10.1177/1082013217697469.

Muñoz J, Ross P, Cracco P. Flora indígena del Uruguay: árboles y arbustos ornamentales. Montevideo: Hemisferio Sur; 1993. 284p.

Osés SM, Marcos P, Azofra P, de Pablo A, Fernández-Muíño MÁ, Sancho MT. Phenolic profile, antioxidant capacities and enzymatic inhibitory activities of propolis from different geographical areas: needs for analytical harmonization. Antioxidants (Basel). 2020;15;9(1):75. Doi: 10.3390/antiox9010075.

Özkök A, Keskin M, Tanuğur Samancı AE, Yorulmaz Önder E, Takma Ç. Determination of antioxidant activity and phenolic compounds for basic standardization of Turkish propolis. Appl Biol Chem. 2021;64(1):37. Doi: 10.1186/s13765-021-00608-3.

Pant K, Thakur M, Chopra HK, Dar BN, Nanda V. Assessment of fatty acids, amino acids, minerals, and thermal properties of bee propolis from Northern India using a multivariate approach. J Food Compost Anal. 2022;111;104624. Doi: 10.1016/j.jfca.2022.104624.

Paulino Zunini M, Rojas C, De Paula S, Elingold I, Alvareda Migliaro E, Casanova MB, Iribarne Restucia F, Aguilera Morales S, Dubin M. Phenolic contents and antioxidant activity in central-southern Uruguayan propolis extracts. J Chil Chem Soc. 2010;55(1):141-6. Doi: 10.4067/S0717-97072010000100033.

QGIS: Un Sistema de Información Geográfica libre y de Código Abierto [Internet]. Vesion 3.16. Hannover: QGIS Association; 2020 [cited 2023 Dec 28]. Available from: http://www.qgis.org

RStudio Team. RStudio: Integrated Development for R [Internet]. Boston: RStudio; 2015 [cited 2023 Dec 28]. Available from: https://bit.ly/3zLyICd

Serra Bonvehí J, Ventura Coll F. Study on propolis quality from China and Uruguay. Z Naturforsch C J Biosci. 2000;55(9-10):778-84. Doi: 10.1515/znc-2000-9-1017.

Sharma S, Vig AP. Evaluation of in vitro antioxidant properties of methanol and aqueous extracts of Parkinsonia aculeata L. leaves. Sci World J. 2013;2013:604865. Doi: 10.1155/2013/604865.

Silva V, Genta G, Möller MN, Masner M, Thomson L, Romero N, Radi R, Fernandes DC, Laurindo FR, Heinzen H, Fierro W, Denicola A. Antioxidant activity of uruguayan propolis: In vitro and cellular assays. J Agric Food Chem. 2011;59(12):6430-7. Doi: 10.1021/jf201032y.

Soós Á, Bódi É, Várallyay S, Molnár S, Kovács B. Element composition of propolis tinctures prepared from Hungarian raw propolis. LWT. 2022;154:112762. Doi: 10.1016/j.lwt.2021.112762.

Soós Á, Bódi É, Várallyay S, Molnár S, Kovács B. Mineral content of propolis tinctures in relation to the extraction time and the ethanol content of the extraction solvent. LWT. 2019;111:719-26. Doi: 10.1016/j.lwt.2019.05.090.

Soto-Vásquez ML. Metabolitos secundarios, cuantificación de fenoles y flavonoides totales de extractos etanólicos de propóleos de tres localidades del Perú. In Crescendo. 2015;6:37-47.

Sousa JP, Furtado NA, Jorge R, Soares AE, Bastos JK. Perfis físico-químico e cromatográfico de amostras de própolis produzidas nas microrregiões de Franca (SP) e Passos (MG), Brasil. Rev bras farmacogn. 2007;17(1). Doi: 10.1590/S0102-695X2007000100017.

Souza EA, Zaluski R, Veiga N, Orsi RO. Effects of seasonal variations and collection methods on the mineral composition of propolis from Apis mellifera Linnaeus Beehives. Braz J Biol. 2016;76(2):396-401. Doi: 10.1590/1519-6984.16714.

Tosic S, Stojanovic G, Mitic S, Pavlovic A, Alagic S. Mineral composition of selected Serbian propolis samples. J Apic Sci. 2017;61(1):5-15. Doi: 10.1515/jas-2017-0001.

Tran CTN, Brooks PR, Bryen TJ, Williams S, Berry J, Tavian F, McKee B, Tran TD. Quality assessment and chemical diversity of Australian propolis from Apis mellifera bees. Sci Rep. 2022;12(1):13574. Doi: 10.1038/s41598-022-17955-w.

Valencia D, Alday E, Robles-Zepeda R, Garibay-Escobar A, Galvez-Ruiz JC, Salas-Reyes M, Jiménez-Estrada M, Velazquez-Contreras E, Hernandez J, Velazquez C. Seasonal effect on chemical composition and biological activities of Sonoran propolis. Food chem. 2012;131(2):645-51. Doi: 10.1016/j.foodchem.2011.08.086.

Wieczorek PP, Hudz N, Yezerska O, Horčinová-Sedláčková V, Shanaida M, Korytniuk O, Jasicka-Misiak I. Chemical variability and pharmacological potential of propolis as a source for the development of new pharmaceutical products. Molecules. 2022;27(5):1600. Doi: 10.3390/molecules27051600.

Zhang Y, Cao C, Yang Z, Jia G, Liu X, Li X, Cui Z, Li A. Simultaneous determination of 20 phenolic compounds in propolis by HPLC-UV and HPLC-MS/MS. J Food Compos Anal. 2023;115:104877. Doi: 10.1016/j.jfca.2022.104877.

Zullkiflee N, Taha H, Usman A. Propolis: Its role and efficacy in human health and diseases. Molecules. 2022;27(18):6120. Doi: 10.3390/molecules27186120.

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Published

2024-02-26

How to Cite

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Cracco P, Cabrera MC, Gallieta G, Saadoun A. Polyphenols and minerals in propolis from different agricultural regions of Uruguay. Agrocienc Urug [Internet]. 2024 Feb. 26 [cited 2024 Jun. 14];28:e1240. Available from: https://agrocienciauruguay.uy/index.php/agrociencia/article/view/1240

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