Fungicide Resistance in Botrytis cinerea in Uruguay

Abstract

Botrytis cinerea causes important losses in various crops and can easily acquire resistance to fungicides. In order to evaluate resistance in Uruguay, the fungicide sensitivity of 169 monosporic isolates from blueberry, tomato, strawberry, vine, rose and eucalyptus from different regions, was assessed. The minimum inhibitory concentration (MIC) of Carbendazim (Carbendazim, 50,0% i.a.), Iprodione (Rovral, 50.0% i.a., BAYER), Pyrimetanil (Mythos, 30.0% i.a., BAYER) and Pyraclostrobin + Boscalid (Bellis, 12.80% y 25.20% i.a., respectively, BASF) was determined. Growth after 48 hours was evaluated on potato dextrose agar (gelatin glucose agar for pirimetanil) with diferent concentrations of fungicide. Populations differing in behavior were found. Resistance to Cabendazim (MIC > 128 mg/l) predominated in all crops samples, except in blueberries. Iprodione MICs ranged from <1 to >16 mg/l, and 30% of the isolates´ MIC was > 8 mg/l. The majority of isolates´ MIC was < 4 mg/l of Boscalid + 1 mg/l of Pyraclostrobin, but for 7% MIC was > 32 mg/l + > 16 mg/l. The host crop had a very significant effect on the sensitivity to fungicides. Isolates from blueberries and eucalyptus were the most sensitive, except when the latter were tested with Carbendazim. Isolates from tomato and rose were the most resistant,and those from strawberry were intermediate.