First report of Neofusicoccum parvum causing stem canker on cannabis in Uruguay




Botryosphaeriaceae, Cannabis sativa, hemp, identification, pathogenicity


In 2018 and 2019, in Uruguay, pests and fungi associated with diseases were surveyed in cannabis (Cannabis sativa) crops in the south of the country. Neofusicoccum sp. was isolated from plants with canker symptoms on stems in 60% of the crops, occupying the third place of the most frequent fungi. In order to determine the causal agent of stem and branch canker in cannabis, three isolates of Neofusicoccum sp. were characterized by morphological and molecular analysis, as well as pathogenicity in cannabis plants. Neofusicoccum parvum was identified as the causal agent of stem canker in cannabis plants in Uruguay.


Download data is not yet available.


Abdollahzadeh J, Zare R, Phillips A. Phylogeny and taxonomy of Botryosphaeria and Neofusicoccum species in Iran, with description of Botryosphaeria scharifii. Nov. Mycologia. 2013;105:210-20. Doi: 10.3852/12-107. DOI:

Abreo E, Martínez S, Bettucci L, Lupo S. Characterization of Botryosphaeriaceae species associated with grapevines in Uruguay. Australas Plant Pathol. 2013;42(3):241-9. Doi: 10.1007/s1331 3-013-0200-8. DOI:

Alberti I, Prodi A, Nipoti P, Grassi G. First report of Nepfusicoccum parvum causing stem and branch canker of Cannabis sativa in Italy. J Plant Dis Prot. 2018;125(3):511-3. Doi: 10.10d07/s41348-018-0174-4. DOI:

Benson DA, Cavanaugh M, Clark K, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW. GenBank. Nucleic Acids Res. 2013;41(D1):D36-42. Doi: 10.1093/nar/gks1195. DOI:

Delgado-Cerrone L, Mondino-Hintz P, Alaniz-Ferro S. Botryosphariaceae species associated with stem canker, die-back and fruit rot on apple in Uruguay. Eur J Plant Pathol. 2017;146(3):637-55. DOI:

Feng C, Villarroel-Zeballos MI, Ficheux PF, Zima H, Dhillon BDS, Correll JC. First report of Neofusicoccum parvum causing dieback and canker disease on hemp in the United States. Plant Dis. 2020;104(11):2754-3089. Doi: 10.1094/PDIS-03-20-0486-PDN. DOI:

Gobi Labs. Analytical Report [Internet]. Wheat Ridge: Gobi Labs; 2020 [cited 2023 Jun 12]. 1p. Report no.: P-3974857-V1. Available from:

Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018;35(6):1547-49. Doi: 10.1093/molbev/msy096. DOI:

Pavlic D, Slippers B, Coutinho TA, Wingfield MJ. Multiple gene genealogies and phenotypic data reveal cryptic species of the Botryosphaeriaceae: a case of study on the Neofusicoccum parvum/N. ribis complex. Mol Phylogenet Evol. 2009;51(5):259-68. Doi: 10.1016/j.ympev.2008.12.017. DOI:

Pennycook S, Samuels G. Botryosphaeria and Fusicoccum species asociated whit ripe fruit rot of Actinidia deliciosa (kiwifruit) in New Zealand. Mycotaxon. 1985;24(24):445-58.

Phillips A, Alvez A,Abdollahzadeh J, Slippers B, Wingfield J, Groenewald J, Crous P. The Botryosphaeriaceae: genera and species know from culture. Stud Mycol. 2013;76:51-167. Doi: 10.3114/sim0021.

Phillips AJL, Alves A, Abdollahzadeh J, Slippers B, Wingfield MJ, Groenewald JZ, Crous PW. The Botryosphaeriaceae: genera and species known from culture. Stud Mycol. 2013;(1):51-167. Doi: 10.3114/sim0021. DOI:

Rousserie G, Collazo D. Prospección de plagas, hongos y oomicetos asociados a enfermedades en Cannabis sativa L. en el sur de Uruguay [grade’s thesis]. Montevideo (UY): Universidad de la República, Facultad de Agronomía; 2022. 174p.

Sector cannabis en Uruguay [Internet]. Montevideo: Uruguay XXI; 2021 [cited 2023 Jun 12]. 28p. Available from:

Sessa L, Abreo E, Bettucci L, Lupo S. Botryosphaeriaceae species associated with wood diseases of stone and pome fruits trees: symptoms and virulence across different hosts in Uruguay. Eur J Plant Pathol. 2016;146(3):519-30. Doi: 10.1007/s1065 8-016-0936-4. DOI:



How to Cite

Sasia E, González Rabelino P, Collazo D, Rousserie G, Silvera Pérez E. First report of Neofusicoccum parvum causing stem canker on cannabis in Uruguay. Agrocienc Urug [Internet]. 2023 Jul. 27 [cited 2024 Jun. 15];27:e1172. Available from:



Plant protection
QR Code


Article metrics
Abstract views
Galley vies
PDF Views
HTML views
Other views

Most read articles by the same author(s)