Detection and characterization of citrus viroid complex in Uruguay

Authors

  • G. Pagliano Facultad de Agronomía, Laboratorio de Biotecnología. Garzón 780.
  • L. Orlando Facultad de Agronomía, Laboratorio de Biotecnología. Garzón 780.
  • A. Gravina Facultad de Agronomía, Laboratorio de Biotecnología. Garzón 780.

DOI:

https://doi.org/10.31285/AGRO.02.1143

Keywords:

citrus viroid complex}, exocortis, molecular diagnostic

Abstract

Atrog citron Arizona 861 (Citrus medica L.) plants were inoculated with bark from different field sources showing dwarfing and bark scaling and form asymptomatic trees. After 3 and 4 months they showed reduced growth, epinasty in leaves, vein necrosis, point necrosis of the petiole and shock symptoms. sPAGE, slot blot and molecular hybridization analysis of nucleic acids allowed to detect the presence of three viroid group, CVd-I; CVd-II and CVd-III first reported in our country, associated with the causal agent of citrus exocortis (CEVd). The viroids from group CVd-IV has not been detected. All the tested field sources showed a mixture of CEVd with viroids of the groups CV-I, CVd-II and CVd-III.

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Published

1998-06-01

How to Cite

1.
Pagliano G, Orlando L, Gravina A. Detection and characterization of citrus viroid complex in Uruguay. Agrocienc Urug [Internet]. 1998 Jun. 1 [cited 2024 Jun. 15];2(1):74-83. Available from: https://agrocienciauruguay.uy/index.php/agrociencia/article/view/1143

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