Identificação e caracterização de peptídeos antimicrobianos no Transcriptoma de Feijoa sellowiana
DOI:
https://doi.org/10.31285/AGRO.29.1556Palavras-chave:
AMPs, goiabeira-serrana, validação gênica, transcriptômica de novo, defensinasResumo
Os peptídeos antimicrobianos (AMPs) são pequenos polipeptídios presentes em uma grande diversidade de organismos filogeneticamente distantes, incluindo plantas. Eles fazem parte da resposta imunológica inata, permitindo uma ação rápida com um custo energético menor do que o sistema imunológico adaptativo. Diversas aplicações desses peptídeos como agentes anti-infecciosos foram demonstradas. Apesar de seu significativo potencial, a bioprospecção de AMPs na flora nativa do Uruguai ainda é incipiente. O objetivo deste estudo foi identificar e caracterizar genes que codificam seis famílias de peptídeos antimicrobianos compreendendo: defensinas, snakins, tioninas, hevein-like, proteínas de transferência de lipídios (LTPs) e ciclotídeos, na espécie frutífera nativa Feijoa sellowiana (Myrtaceae), comumente conhecida como goiabeira-serrana. A busca foi realizada usando BLAST, baseada em um transcriptoma de novo de folha e flor, utilizando seqüências de referência. As relações entre F. sellowiana e sequências de proteínas de referência foram caracterizadas por meio de alinhamentos múltiplos e análise filogenética. A estrutura exon-íntron e isoformas de AMPs de F. sellowiana foram analisadas por amplificação por PCR em DNA genômico. Este estudo identificou 23 defensinas, 49 snakins, 7 tioninas, 12 hevein-like, 87 LTPs; nenhuma evidência de transcrições de ciclotídeo foi encontrada. A estrutura exon-íntron esperada foi confirmada para cinco seqüências, pertencentes às famílias defensinas, esnaquinas e hevein-like.
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