Ecophysiological Response to Increased Photosynthetic Photon Flux Density (PPFD) in Handroanthus chrysanthus (Jacq.) S.O. Grose and Swietenia macrophylla King Seedlings Under Amazonian Conditions
DOI:
https://doi.org/10.31285/AGRO.30.1807Keywords:
gas exchange, light intensity, photosynthesis, reforestationAbstract
Handroanthus chrysanthus and Swietenia macrophylla are ecologically and economically significant tree species whose populations have declined due to high demand for their timber, adversely affecting their natural regeneration. This study aimed to evaluate the ecophysiological response to increased photosynthetic photon flux density (PPFD) in H. chrysanthus and S. macrophylla seedlings under Amazonian conditions. Assimilation measurements (A) were performed using a portable iFL-LCpro-SD system. The evaluation of A in response to increased PPFD ranged from 25 to 1800 μmol m⁻²s⁻¹. Water use efficiency (WUE) was calculated as the ratio between A and the transpiration rate (E). S. macrophylla exhibited a significantly higher maximum photosynthetic assimilation rate (Amax, 9.4 ± 0.52 µmol CO₂ m⁻² s⁻¹) compared to H. chrysanthus (6.18 ± 0.17 µmol CO₂ m⁻² s⁻¹, p < 0.05), indicating greater carbon fixation efficiency. S. macrophylla showed a maximum WUE (WUEmax) of 13.17 ± 0.24 µmol mmol⁻¹ at 600 μmol m⁻² s⁻¹ PPFD, while H. chrysanthus reached its WUEmax (6.16 ± 0.23 µmol mmol⁻¹) at 750 μmol m⁻² s⁻¹ PPFD. These results suggest that S. macrophylla exhibits higher WUE under high irradiance conditions, potentially due to more efficient stomatal regulation and an optimized balance between carbon fixation and water loss via transpiration.
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Amaral, G. C., Pezzopane, J. E. M., de Souza Nóia Júnior, R., Fonseca, M. D. S., Martínez, M. F., de Oliveira Gomes, V., Toledo, J. V., Pezzopane, J. R. M., & Martín, R. T. (2023). Climate change and the growth of Amazonian species seedlings: An ecophysiological approach to Euterpe oleracea. New Forests, 54(2), 269-287. https://doi.org/10.1007/s11056-022-09921-1
Ávila-Lovera, E., & Tezara, W. (2018). Water-use efficiency is higher in green stems than in leaves of a tropical tree species. Trees, 32(6), 1547-1558. https://doi.org/10.1007/s00468-018-1732-x
Azevedo, G. F. C., & Marenco, R. A. (2012). Growth and physiological changes in saplings of Minquartia guianensis and Swietenia macrophylla during acclimation to full sunlight. Photosynthetica, 50(1), 86-94. https://doi.org/10.1007/s11099-012-0001-2
Cao, Y., Li, J., Li, S., & Zhou, B. (2024). The effects of long-term precipitation exclusion on leaf photosynthetic traits, stomatal conductance, and water use efficiency in Phyllostachys edulis. Forests, 15(5), Article 849. https://doi.org/10.3390/f15050849
Cordeiro, Y. E. M., Pinheiro, H. A., dos Santos Filho, B. G., Corrêa, S. S., e Silva, J. R. R., & Dias-Filho, M. B. (2009). Physiological and morphological responses of young mahogany (Swietenia macrophylla King) plants to drought. Forest Ecology and Management, 258(7), 1449-1455. https://doi.org/10.1016/j.foreco.2009.06.054
Dai, Y., Guo, Z., Guo, X., Deng, R., Li, L., Fan, T., Cui, K., & Pan, T. (2024). Plastic particles and fluorescent brightener co-modify Chlorella pyrenoidosa photosynthesis and a machine learning approach predict algae growth. Journal of Hazardous Materials, 477, Article 135406. https://doi.org/10.1016/j.jhazmat.2024.135406
de Oliveira Neves, F., Salgado, E. G., & Lira, J. M. S. (2024). Energetic sustainability in the Amazon region: Exploring impacts, environmental solutions, and rebound effect analysis. Environmental Development, 51, Article 101043. https://doi.org/10.1016/j.envdev.2024.101043
Debiasi, T. V., Calzavara, A. K., Gomes, D. G., Andreas, I. R., Rondina, A. B. L., Duarte, K. E., Pereira, R. M., Batista, B. L., Pimenta, J. A., Seabra, A. B., Centeno, D. C., Gaspar, M., & Oliveira, H. C. (2024). Influence of light intensity on the responses of seedlings of neotropical tree species to nitrogen source. Environmental and Experimental Botany, 228, Article 106007. https://doi.org/10.1016/j.envexpbot.2024.106007
Dumais, D., Raymond, P., & Champagne, E. (2025). Translocated southern seedlings perform as well as local provenances: Insights from an ecophysiological monitoring under varying cutting modalities. New Forests, 56(1), 1-25. https://doi.org/10.1007/s11056-024-10089-z
Espinosa, C. I., Camarero, J. J., & Gusmán, A. A. (2018). Site-dependent growth responses to climate in two major tree species from tropical dry forests of southwest Ecuador. Dendrochronologia, 52, 11-19. https://doi.org/10.1016/j.dendro.2018.09.004
García-Quintana, Y., Arteaga-Crespo, Y., Torres-Navarrete, B., Robles-Morillo, M., Bravo-Medina, C., & Sarmiento-Rosero, A. (2020). Ecological quality of a forest in a state of succession based on structural parameters: A case study in an evergreen Amazonian-Andean forest, Ecuador. Heliyon, 6(7), Article e04592. https://doi.org/10.1016/j.heliyon.2020.e04592
He, T., Marco, J., Soares, R., Yin, Y., & Wiedenhoeft, A. C. (2020). Machine Learning Models with Quantitative Wood Anatomy Data Can Discriminate between Swietenia macrophylla and Swietenia mahagoni. Forests, 11(1), Article 36. https://doi.org/10.3390/f11010036
Héctor, E., Cevallos, D., Corozo, L., Macías, F., & Fosado, O. (2024). Development of a protocol for the micropropagation of two forest species threatened with extinction in Ecuador. Plant Cell, Tissue and Organ Culture, 159(2). https://doi.org/10.1007/s11240-024-02864-9
Herrera-Feijoo, R. J., Torres, B., López-Tobar, R., Tipán-Torres, C., Toulkeridis, T., Heredia-R, M., & Mateo, R. G. (2023). Modelling climatically suitable areas for mahogany (Swietenia macrophylla King) and their shifts across neotropics: The role of protected areas. Forests, 14(2), Article 385. https://doi.org/10.3390/f14020385
Jhou, H. C., Wang, Y. N., Wu, C. S., Yu, J. C., & Chen, C. I. (2017). Photosynthetic gas exchange responses of Swietenia macrophylla King and Melia azedarach L. plantations under drought conditions. Botanical Studies, 58(1), Article 57. https://doi.org/10.1186/s40529-017-0212-8
Jin, C., Zha, T., Bourque, C. P., Di, K., Zhang, W., Jiao, Y., Fan, Z., & Hu, Z. (2024). Water use efficiency in tropical plants based on a set of newly created leaf photosynthesis-related parameters. The Science of the Total Environment, 957, Article 177657. https://doi.org/10.1016/j.scitotenv.2024.177657
Kabala, J. P., Niccoli, F., Altieri, S., Liyaqat, I., & Battipaglia, G. (2024). Distinct responses of climate-growth and iWUE in Fagus sylvatica L. at two low elevation sites in southern Italy. Journal of Forestry Research, 36(1), 1-17. https://doi.org/10.1007/s11676-024-01788-6
Kieffer, C., Kaur, N., Li, J., Matamala, R., Fay, P. A., & Hui, D. (2024). Photosynthetic responses of switchgrass to light and CO2 under different precipitation treatments. Global Change Biology Bioenergy, 16, Article e13138. https://doi.org/10.1111/gcbb.13138
Kübler, D., Hildebrandt, P., Günter, S., Stimm, B., Weber, M., Muñoz, J., Cabrera, O., Zeilinger, J., Silva, B., & Mosandl, R. (2020). Effects of silvicultural treatments and topography on individual tree growth in a tropical mountain forest in Ecuador. Forest Ecology and Management, 457, Article 117726. https://doi.org/10.1016/j.foreco.2019.117726
Laisk, A. (1997). Kinetics of photosynthesis and photorespiration in C3 plants. Nauka, 198, 243-251.
Leyerer, L., & Katzensteiner, K. (2025). Effects of substrate on ecophysiology of young silver fir and Norway spruce growing on shallow calcareous soils. Forest Ecology and Management, 578, Article 122477. https://doi.org/10.1016/j.foreco.2024.122477
Liu, C., Peltoniemi, M., Alekseychik, P., Mäkelä, A., & Hölttä, T. (2025). A coupled model of hydraulic eco-physiology and cambial growth - accounting for biophysical limitations and phenology improves stem diameter prediction at high temporal resolution. Plant, Cell & Environment, 48(2), 1344-1365. https://doi.org/10.1111/pce.15239
Melo, A., Nicodemos, J., Lopes, R. F., & Santos, M. G. (2025). Seasonal balance of whole plant non-structural carbohydrates in a deciduous species with green stem in a dry tropical forest. Journal of Arid Environments, 227, Article 105324. https://doi.org/10.1016/j.jaridenv.2025.105324
Ministerio del Ambiente, Agua y Transición Ecológica. (2024). Sistematización del estudio poblacional de especies forestales CITES: Swietenia macrophylla (ahuano) y Cedrela odorata (cedro). Universidad Técnica Particular de Loja.
Oluborode, J., Chadzinikolau, T., Formela-Luboińska, M., Ye, Z. P., & Robakowski, P. (2025). Adaptive significance of age- and light-related variation in needle structure, photochemistry, and pigments in evergreen coniferous trees. Photosynthesis Research, 163(1), Article 3. https://doi.org/10.1007/s11120-024-01125-2
Rosati, A., Wolz, K. J., Murphy, L., Ponti, L., & Jose, S. (2020). Modeling light below tree canopies overestimates net photosynthesis and radiation use efficiency in understory crops by averaging light in space and time. Agricultural and Forest Meteorology, 284, Article 107892. https://doi.org/10.1016/j.agrformet.2019.107892
Sánchez Amboage, E., Sánchez Cevallos, R. E., Bustamante Sánchez, N. S., & Mora Jácome, V. L. (2016). Turismo experiencial a través del florecimiento de los guayacanes del sur de Ecuador: Un análisis desde su comunicación. In J. R. Araújo, V. A. Martínez Fernández, M. M. Rodríguez, I. P. Rivera, J. Y. Quichimbo & E. Sánchez Amboage (Eds.), De los medios y la comunicación de las organizaciones a las redes de valor (pp. 566-579). Universidad Técnica Particular de Loja. https://dialnet.unirioja.es/servlet/articulo?codigo=6075397&info=resumen&idioma=ENG
Schmiege, S. C., Sharkey, T. D., Walker, B., Hammer, J., & Way, D. A. (2023). Laisk measurements in the nonsteady state: Tests in plants exposed to warming and variable CO2 concentrations. Plant Physiology, 193(2), 1045-1057. https://doi.org/10.1093/plphys/kiad305
Sembada, A. A., Faizal, A., & Sulistyawati, E. (2024). Photosynthesis efficiency as key factor in decision-making for forest design and redesign: A systematic literature review. Ecological Frontiers, 44(6), 1128-1139. https://doi.org/10.1016/j.ecofro.2024.07.008
Song, H., Zhong, T., Zhu, J., & Yan, T. (2025). Higher risk of hydraulic dysfunction and carbohydrate depletion of declining Larix principis-rupprechtii trees. Ecological Processes, 14(1), 1-10. https://doi.org/10.1186/s13717-024-00567-9
Tang, X., Zhao, J., Zhou, J., Zhu, Q., Sheng, X., & Yue, C. (2024). Elevated CO2 Shifts Photosynthetic Constraint from Stomatal to Biochemical Limitations During Induction in Populus tomentosa and Eucalyptus robusta. Plants (Basel), 14(1), Article 47. https://doi.org/10.3390/plants14010047
Trugman, A. T., & Anderegg, L. D. L. (2025). Source vs sink limitations on tree growth: From physiological mechanisms to evolutionary constraints and terrestrial carbon cycle implications. The New Phytologist, 245(3), 966-981. https://doi.org/10.1111/nph.20294
Ureta-Leones, D., Artega-Crespo, Y., García-Quintana, Y., & Arellano-Reinoso, K. (2024). Respuesta fotosintética de Guadua angustifolia Kunth y Bambusa vulgaris Schrad. ex J.C. Wendl. a diferentes intensidades de luz. Revista Cubana de Ciencias Forestales, 12(3), Article e869. http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S2310-34692024000300007&lng=en&tlng=es
Valladares, F., & Niinemets, Ü. (2008). Shade tolerance, a key plant feature of complex nature and consequences. Annual Review of Ecology, Evolution, and Systematics, 39, 237-257. https://doi.org/10.1146/annurev.ecolsys.39.110707.173506
Xu, F., Guo, W., Xu, W., Wei, Y., & Wang, R. (2009). Leaf morphology correlates with water and light availability: What consequences for simple and compound leaves? Progress in Natural Science, 19(12), 1789-1798. https://doi.org/10.1016/j.pnsc.2009.10.001
Yan, J., Zhu, C., Liu, W., Luo, F., Mi, J., Ren, Y., Li, J. & Sang, T. (2015), High photosynthetic rate and water use efficiency of Miscanthus lutarioriparius characterize an energy crop in the semiarid temperate region. Global Change Biology Bioenergy, 7, 207-218. https://doi.org/10.1111/gcbb.12118
Zhang, S., Wang, X., Huang, Z., Bao, Y. T., Jiang, J., & Liu, Z. (2025). Quercus acutissima exhibits more adaptable water uptake patterns in response to seasonal changes compared to Pinus massoniana. Forest Ecosystems, 12, Article 100255. https://doi.org/10.1016/j.fecs.2024.100255
Zhao, N., Lu, S., Li, S., Li, B., Yu, X., & Xu, X. (2025). Enhancing the water use efficiency model predictions for Platycladus orientalis and Quercus variabilis: Integrating the dynamics of carbon dioxide concentration and soil water availability. The Science of the total environment, 959, Article 178179. https://doi.org/10.1016/j.scitotenv.2024.178179
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