Advances in the development of a biofungicide

Physical-chemical characterization and antifungal activity of propolis

  • Irene Laura Cibanal Laboratorio de Estudios Apícola (LabEA) – Departamento de Agronomía – Universidad Nacional del Sur. Calle San Andrés 800, Barrio Altos del Palihue, Ciudad de Bahía Blanca, Provincia de Buenos Aires, Argentina. https://orcid.org/0000-0001-5224-5462
  • Leticia A. Fernández Laboratorio de Estudios Apícola (LabEA) – Departamento de Agronomía – Universidad Nacional del Sur. Calle San Andrés 800, Barrio Altos del Palihue, Ciudad de Bahía Blanca, Provincia de Buenos Aires, Argentina. https://orcid.org/0000-0003-3785-8003
  • Gabriela Krepper Instituto de Química del Sur (INQUISUR) – Departamento de Química - Universidad Nacional del Sur. Calle Av. Alem 1253, Ciudad de Bahía Blanca, Provincia de Buenos Aires, Argentina. https://orcid.org/0000-0002-0674-6888
  • Cecilia Noemí Pellegrini Laboratorio de Estudios Apícola (LabEA) – Departamento de Agronomía – Universidad Nacional del Sur. Calle San Andrés 800, Barrio Altos del Palihue, Ciudad de Bahía Blanca, Provincia de Buenos Aires, Argentina. https://orcid.org/0000-0002-6137-2700
  • Liliana María Gallez Laboratorio de Estudios Apícola (LabEA) – Departamento de Agronomía – Universidad Nacional del Sur. Calle San Andrés 800, Barrio Altos del Palihue, Ciudad de Bahía Blanca, Provincia de Buenos Aires, Argentina. https://orcid.org/0000-0002-2706-8908
Keywords: apis mellifera, biofungicide, flavonoids, Penicillium sp., phenols

Abstract

Propolis is a viable alternative for chemical control of plant pathogens due to its antimicrobial properties. The present study was conducted to determine the sensorial, physical and chemical characteristics of four propolis from Argentina, and their respective hydroalcoholic extracts. In vitro antifungal activity of hydroalcoholic propolis extracts against Penicillium sp. that cause the decay of garlic (Allium sativum) was also studied. All propolis samples were composed of irregular fragments. The aroma was mainly resinous. The predominant colour was brown. The analysis of the raw samples showed differences in the parameters wax content (9.43-31.16 %), loss by heating (1.04-1.46 %), ashes (0.98-7.1 8 %) and melting point (63.16-67.5 °C). The hydroalcoholic extracts of propolis also differ in the dry residue (5.50-9.30 %), content of phenols (23.44-53.91 mg eq galic/g propolis), total flavonoids (11.23-15.88 mg eq quercetin/g propolis) and oxidation index (2.25-4.33 seconds). The evaluation of the antifungal activity demonstrated that all the treatments containing propolis had an inhibitory effect over 99 % on fungi colony-forming units’ development. Although the physical and chemical study showed significant differences between propolis samples, all exhibited excellent antifungal activity against Penicillium sp. This demonstrates the potential of different propolis for the development of a biofungicide for agricultural use.

Published
2019-10-23
How to Cite
1.
Cibanal I, Fernández L, Krepper G, Pellegrini C, Gallez L. Advances in the development of a biofungicide. Agrociencia Uruguay [Internet]. 23Oct.2019 [cited 27Feb.2020];23(2):1-10. Available from: http://agrocienciauruguay.uy/ojs/index.php/agrociencia/article/view/83
Section
Plant protection