dentification of Earthworm Species in Uruguay Based on Morphological and Molecular Methods

  • Gabriella Jorge Escudero Universidad de la República, Facultad de Agronomía, Departamento de Suelos y Aguas. Garzón 780, 12900 Montevideo, Uruguay. https://orcid.org/0000-0002-2961-8846
  • Jan Lagerlöf Universidad Sueca de Agricultura (SLU), Departamento de Ecología. P.O Box 7044, SE-75007, Uppsala, Suecia. https://orcid.org/0000-0003-2242-806X
  • Claudio Martínez Debat Universidad de la República, Facultad de Ciencias, Sección Bioquímica. Iguá 4225 Esq. Mataojo , 11400 Montevideo, Uruguay. https://orcid.org/0000-0003-3015-0117
  • Carlos Alberto Pérez Universidad de la República, Facultad de Agronomía, EEMAC, Departamento de Protección Vegetal. Ruta 3 km 363, 60.000 Paysandú, Uruguay. https://orcid.org/0000-0003-1907-6745
Keywords: COI, sequencing, native and exotic earthworms

Abstract

Molecular techniques could aid earthworm species identification, especially when morphological characters are not taxonomically informative, or difficult to discern. No previous study has investigated molecular-based methods for earthworm taxonomy in Uruguay. The present study aimed to make a first approach using DNA barcoding as a tool to smoothen the way towards determining the earthworm richness in Uruguay. This study was based on an earthworm collection, identified both by morphological characters and molecular techniques, from samplings from different agricultural soils in Montevideo and Paysandú, Uruguay. Adult individuals were identified by external morphology following available descriptions of regional earthworms. From each morphological group a representative sample was selected for genomic DNA extraction, mitochondrial COI region amplification and sequencing. Sequences obtained were subject to BLAST searches and compared to sequences available in GenBank. Eight out of 11 sequenced exotic species were fully identified and matched morphological characters and molecular information; two were less consistent, with lower sequence similarity percentage; and one could not be fully identified due to lack of close related sequences in GenBank. While most exotic species had representative sequences annotated in GenBank, this was only the case for one native species, highlighting the need to develop this important area at a regional level. This study could kick-start an innovative research program, since there are limited records of earthworm samplings in Uruguay and no identification of species by DNA sequences from national studies.

Published
2019-05-23
How to Cite
1.
Jorge Escudero G, Lagerlöf J, Martínez Debat C, Pérez C. dentification of Earthworm Species in Uruguay Based on Morphological and Molecular Methods. Agrociencia Uruguay [Internet]. 23May2019 [cited 20Nov.2019];23(1):1-0. Available from: http://agrocienciauruguay.uy/ojs/index.php/agrociencia/article/view/58
Section
Plant protection