Cytotoxic effect of Senecio madagascariensis (Asteraceae) extracts on cancer derived cell lines

  • Alejandra Mondino Universidad de la República, Facultad de Veterinaria, Unidad Académica de Toxicología, Montevideo, Uruguay https://orcid.org/0000-0002-8746-2464
  • Kevin Yaneselli Universidad de la República, Facultad de Veterinaria, Unidad Académica de Inmunología, Montevideo, Uruguay https://orcid.org/0000-0002-4602-5698
  • Ana Ingold Universidad de la República, Facultad de Veterinaria, Unidad Académica de Toxicología, Montevideo, Uruguay https://orcid.org/0000-0002-1429-5992
  • Carolina Echeverry Instituto de Investigaciones Biológicas Clemente Estable, Departamento de Neuroquímica, Montevideo, Uruguay https://orcid.org/0000-0002-3953-4814
  • Sofia Raffaelli Universidad de la República, Facultad de Química, Laboratorio de Farmacognosia y Productos Naturales, Montevideo, Uruguay https://orcid.org/0000-0002-7194-0855
  • Álvaro Vázquez Universidad de la República, Facultad de Química, Laboratorio de Farmacognosia y Productos Naturales, Montevideo, Uruguay https://orcid.org/0000-0002-6516-2225
  • Carmen García y Santos Universidad de la República, Facultad de Veterinaria, Unidad Académica de Toxicología, Montevideo, Uruguay https://orcid.org/0000-0002-2485-0099
Keywords: anti-proliferative activity, cytotoxic activity, phytomedicine, senecio madagascariensis, medicinal plants

Abstract

Species of the genus Senecio have been traditionally employed with medical purposes and it has been demonstrated that some of them have anti-proliferative activity on cancer-derived cell lines. In South America S. madagascariensis is one of the most important representatives of the genus. Almost all species contain pyrrolizidine alkaloids (PA), substances that can lead to hepatic damage. Thus, the aim of this work was to determine the cytotoxic activity of a low PAs extract of S. madagascariensis on a cancer-derived cell line. A chloroform extract from plant material was produced and it was demonstrated to have a PAs concentration lower than 200 μg/g (w/w). Two stock solutions were made with DMSO and acetone as solvents and were diluted in culture medium (DMEM). Cells from urinary bladder transitional cell carcinoma T24 were cultured in 96 well plates (104 cells/well) and incubated for 24 hours with dilutions of the extracts at the following concentrations, 0.5 mg/mL, 0.25 mg/mL, 0.125 mg/mL and 0.06 mg/mL. Cell viability was evaluated by the MTT colorimetric technique. Both solutions of S. madagascariensis extracts (acetone and DMSO) showed cytotoxic activity. In both cases, the degree of cytotoxicity was dependent on the concentration of the solution.

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References

Achika J, Arthur DE, Gerald I, Adedayo A. A Review on the Phytoconstituents and Related Medicinal Properties of Plants in the Asteraceae Family. IOSR J Appl Chem [Internet]. 2014 [cited 2021 Oct 22];7(8):1-8. Available from: https://bit.ly/3B9FthT.

Bassey ME, Johnny I, Umoh OT, Douglas FT. Phytomedicinal potentials of Species of Nephrolepis. World J Pharm Res. 2020;9(4):1400-10.

Caniato R, Tabacchi R, Tsoupras G, Bicchi C. Capillary Gas Chromatography/Positive and Negative Ion Chemical Ionization Mass Spectrometry on Pyrrolizidine Alkaloids of Senecio inaequidens Using Ammonia and Hydroxyl Ions as the Reagent Species. J Nat Prod [Internet]. 1989 [cited 2021 Oct 22];52(1):32-41. Doi: 10.1021/np50061a002.

Conforti F, Loizzo MR, Statti G, Houghton P, Menichini F. Biological properties of different extracts of two Senecio species. Int J Food Sci Nutr. 2006;57(1-2):1-8.

Ferreira F, Moyna P. Rapid determination of solanum glycoalkaloids by thin-layer chromatographic scanning. J Cromatography. 1993;653:380-4.

González Urones J, Basabe Barcala P, Sánchez Marcos I, Fernández Moro R, López Esteban M, Fernandez Rodriguez A. Pyrrolizidine alkaloids from Senecio gallicus and Senecio donifolius. Phytochemistry. 1988;27(5):1507-10.

Heinrich M, Robles M, West JE, Ortiz de Montellano BR, Rodriguez E. Ethnopharmacology of Mexican Asteraceae (Compositae). Annu Rev Pharmacol Toxicol [Internet]. 1998 [cited 2021 Oct 22];38(1):539-65. Doi: 10.1146/annurev.pharmtox.38.1.539.

Hubinger Tokarnia C, Döbereiner J, Vargas Peixoto P. Plantas tóxicas do Brasil. Rio de Janeiro: Helianthus; 2000. 309p.

Jeffrey C. Compositae: Introduction with Keys to Tribes. In: Kadereit JW, Jeffrey C, editors. Families and Genera of Vascular Plants [Internet]. Vol. VIII, Flowering Plants, Eudicots, Asterales. Berlin: Springer-Verkag; 2007 [cited 2021 Oct 22]. p. 61-87. (Families and Genera of Vascular Plants). Doi: 10.1007/978-3-540-31051-8.

Loizzo MR, Tundis R, Statti GA, Menichini F. Jacaranone: a cytotoxic constituent from Senecio ambiguus subsp. ambiguus (Biv.) DC. against renal adenocarcinoma ACHN and prostate carcinoma LNCaP cells. Arch Pharm Res. 2007;30(6):701-7.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1-2):55-63.

Omokhua-Uyi AG, Van Staden J. Phytomedicinal relevance of South African Cucurbitaceae species and their safety assessment: a review. J Ethnopharmacol [Internet]. 2020 [cited 2021 Oct 22];259:112967. Doi: 10.1016/j.jep.2020.112967.

Peris JB, Stübing G, Figuerola R, Morán MJ. Guía de las plantas medicinales de la Comunidad Valenciana. Valencia : Las Provincias; 1996. 416p.

Preliasco M, Gardner D, Moraes J, González AC, Uriarte G, Rivero R. Senecio grisebachii Baker: Pyrrolizidine alkaloids and experimental poisoning in calves. Toxicon. 2017;133:68-73.

Preliasco M, Rivero R. Poisoning of Cattle by Senecio Spp. in Uruguay. In: Riet-Correa F, Pfister J, Schild AL, Wierenga T, editors. Poisoning by plants, mycotoxins and related toxins. Cambridge (MA): CABI Publishing; 2009. p. 199-207.

Rastogi RP, Dhawan BN. Anticancer and antiviral activities in indian medicinal plants: a review. Drug Dev Res. 1990;19(1):1-12.

Solecki RS. Shanidar IV, a Neanderthal Flower Burial in Northern Iraq. Science [Internet]. 1975 [cited 2021 Oct 22];190(4217):880-1. Doi: 10.1126/science.190.4217.880.

Stegelmeier BL. Pyrrolizidine alkaloid-containing toxic plants (Senecio, Crotalaria, Cynoglossum, Amsinckia, Heliotropium, and Echium spp.). Vet Clin North Am Food Anim Pract [Internet]. 2011 [cited 2021 Oct 22];27(2):419-28, ix. Doi: 10.1016/j.cvfa.2011.02.013.

Stevigny C, Bailly C, Quetin-Leclercq J. Cytotoxic and Antitumor Potentialities of Aporphinoid Alkaloids. Curr Med Chem Agents [Internet]. 2005 [cited 2021 Oct 22];5(2):173-82. Doi: 10.2174/1568011053174864.

Tamokou JDD, Mbaveng AT, Kuete V. Antimicrobial Activities of African Medicinal Spices and Vegetables. In: Kuete V, editor. Medicinal Spices and Vegetables from Africa: Therapeutic Potential Against Metabolic, Inflammatory, Infectious and Systemic Diseases [Internet]. London: Elsevier; 2017 [cited 2021 Oct 22]. p. 207-37. Doi: 10.1016/B978-0-12-809286-6/00008-X.

Toyang NJ, Verpoorte R. A review of the medicinal potentials of plants of the genus Vernonia (Asteraceae). J Ethnopharmacol [Internet]. 2013 [cited 2021 Oct 22];146(3):681-723. Doi: 10.1016/j.jep.2013.01.040.

Tundis R, Loizzo MR, Bonesi M, Menichini F, Dodaro D, Passalacqua NG, Statti G, Menichini F. In vitro cytotoxic effects of Senecio stabianus Lacaita (Asteraceae) on human cancer cell lines. Nat Prod Res. 2009;23(18):1707-18.

Verschaeve L, Van Staden J. Mutagenic and antimutagenic properties of extracts from South African traditional medicinal plants. J Ethnopharmacol. 2008;119(3):575-87.

Villalba J, Fernández G. Otra flor amarilla peligrosa: Senecio madagascariensis. Rev Plan Agropecu. 2007;123:46-8.

Wang CM, Yang H, Wei YM, Jia ZJ, Zheng RL. In vitro effects on proliferation, telomerase activity and apoptosis of an eremophilanoid sesquiterpene from Senecio oldhamianus Maxim in cultured human tumor cell lines. Pharmazie. 2004;59(10):802-6.

Yaneselli KM, Kuhl CP, Terraciano PB, de Oliveira FS, Pizzato SB, Pazza K, Magrisso AB, Torman V, Rial A, Moreno M, Llambí S, Cirne-Lima E, Maisonnave J. Comparison of the characteristics of canine adipose tissue-derived mesenchymal stem cells extracted from different sites and at different passage numbers. J Vet Sci. 2018;19(1):13-20.

Published
2021-11-25
How to Cite
1.
Mondino A, Yaneselli K, Ingold A, Echeverry C, Raffaelli S, Vázquez Álvaro, García y Santos C. Cytotoxic effect of Senecio madagascariensis (Asteraceae) extracts on cancer derived cell lines. Agrociencia Uruguay [Internet]. 25Nov.2021 [cited 20Jan.2022];26(1):e425. Available from: http://agrocienciauruguay.uy/ojs/index.php/agrociencia/article/view/425
Section
Animal production and pastures